| Rank | Name | Time/Score | Start | Year |
|---|---|---|---|---|
| 1 | Amy Zhao '14 | 24.94 | R | 2014 |
| 2 | Allyson Ty '18 | 25.73 | R | 2018 |
| 3 | Grace Hitchcock '20 | 26.05 | R | 2020 |
| 4 | Annie Glancy '09 | 26.68 | R | 2009 |
| 5 | Katie Swan '23 | 26.83 | R | 2020 |
| 6 | Elizabeth Duserick '16 | 26.85 | R | 2016 |
| 7 | Graeleigh Jones '21 | 26.87 | R | 2019 |
| 8 | Megan Ramsey '00 | 26.93 | R | 2000 |
| 9 | Sarah Demers '03 | 26.94 | R | 2003 |
| 10 | Elizabeth Tran '19 | 27.04 | R | 2017 |
| 11 | Julia Torabi '12 | 27.34 | R | 2010 |
| 12 | Jessica Schoen '99 | 27.46 | R | 1998 |
| 13 | Miranda An '15 | 27.60 | R | 2012 |
| 14 | Kealy O'Connor '96 | 27.66 | R | 1995 |
| 15 | Greta Martin '09 | 27.78 | R | 2007 |
| 16 | Gwen Empie '21 | 27.87 | R | 2020 |
| 17 | Grace Hwang '22 | 27.90 | R | 2020 |
| 18 | Emily Brenner '06 | 27.97 | R | 2005 |
| 19 | Sarah Ferranti '01 | 28.18 | R | 2001 |
| 20 | Alex Techet '91 | 28.61 | R | 1991 |