| Rank | Name | Time/Score | Start | Year |
|---|---|---|---|---|
| 1 | Hee-Jin Chang '05 | 51.01 | R | 2004 |
| 2 | Allyson Ty '18 | 51.23 | R | 2015 |
| 3 | Amy Zhao '14 | 51.23 | R | 2014 |
| 4 | Danielle Liu '14 | 51.69 | R | 2014 |
| 5 | Kaitlin Simpson '14 | 51.85 | R | 2014 |
| 6 | Sydney Freas '01 | 52.37 | R | 1998 |
| 7 | Veronika Kisova '23 | 52.97 | R | 2020 |
| 8 | Erika Roddy '07 | 53.30 | R | 2006 |
| 9 | Kate Page '04 | 53.39 | R | 2001 |
| 10 | Elizabeth Tran '19 | 53.53 | R | 2019 |
| 11 | Graeleigh Jones '21 | 53.63 | R | 2020 |
| 12 | Mary Kate Turk '22 | 53.66 | R | 2019 |
| 13 | Alex Doty '05 | 53.66 | R | 2005 |
| 14 | Jess Gearan '18 | 53.91 | R | 2015 |
| 15 | Teri Faller '11 | 53.93 | R | 2011 |
| 16 | Kristen Faulkner '11 | 54.23 | R | 2008 |
| 17 | Miranda An '15 | 54.30 | R | 2012 |
| 18 | Katie Faulkner '06 | 54.50 | R | 2004 |
| 19 | Katherine Sweetser '17 | 54.57 | R | 2016 |
| 20 | Peyton Morss '10 | 54.58 | R | 2009 |
| 21 | Salena Casha '09 | 54.66 | R | 2009 |
| 22 | Margaret Welles '96 | 54.71 | R | 1995 |
| 23 | Ashley Vensel '22 | 54.73 | R | 2020 |
| 24 | Sofia Smirnov '22 | 54.75 | R | 2020 |
| 25 | Lexi Moroney '11 | 54.78 | R | 2010 |