| Rank | Name | Time/Score | Start | Year |
|---|---|---|---|---|
| 1 | Hee-Jin Chang '05 | AASCH 23.19 | F | 2002 |
| 2 | Amy Zhao '14 | AA 23.44 | F | 2014 |
| 3 | Allyson Ty '18 | 23.91 | F | 2015 |
| 4 | Sydney Freas '01 | 24.01 | F | 2001 |
| 5 | Kaitlin Simpson '14 | 24.41 | F | 2013 |
| 6 | Erika Roddy '07 | 24.56 | F | 2006 |
| 7 | Peyton Morss '10 | 24.82 | F | 2008 |
| 8 | Elizabeth Duserick '16 | 24.98 | F | 2016 |
| 9 | Sasha Newton '16 | 25.05 | F | 2016 |
| 10 | Hailey Novis '13 | 25.17 | F | 2010 |
| 11 | Danielle Liu '14 | 25.18 | F | 2014 |
| 12 | Abby Ryan '21 | 25.18 | F | 2018 |
| 13 | Kerryn O'Connor '01 | 25.24 | F | 2001 |
| 14 | Mary Kate Turk '22 | 25.36 | F | 2020 |
| 15 | Sisi Ruan '07 | 25.37 | F | 2006 |
| 16 | Lexi Moroney '11 | 25.46 | F | 2009 |
| 17 | Sarah Demers '03 | 25.49 | F | 2003 |
| 18 | Kealy O'Connor '96 | 25.50 | F | 1994 |
| 19 | Christina Richardson '98 | 25.53 | F | 1997 |
| 20 | Miranda An '15 | 25.55 | F | 2015 |
| 21 | Emily Brenner '06 | 25.56 | F | 2006 |
| 22 | Juliette Farmer '18 | 25.59 | F | 2015 |
| 23 | Kaitlin Fanikos '11 | 25.62 | F | 2010 |
| 24 | Mel Lind '98 | 25.63 | F | 1996 |
| 25 | Alyssa Hill '06 | 25.63 | F | 2005 |