| Rank | Name | Time/Score | Start | Year |
|---|---|---|---|---|
| 1 | Hee-Jin Chang '05 | AASCH 50.57 | F | 2002 |
| 2 | Amy Zhao '14 | 51.39 | F | 2014 |
| 3 | Kaitlin Simpson '14 | 52.16 | F | 2014 |
| 4 | Sydney Freas '01 | 52.50 | F | 2001 |
| 5 | Allyson Ty '18 | 52.58 | F | 2017 |
| 6 | Danielle Liu '14 | 52.81 | F | 2013 |
| 7 | Erika Roddy '07 | 53.52 | F | 2006 |
| 8 | Peyton Morss '10 | 53.64 | F | 2008 |
| 9 | Jess Gearan '18 | 53.76 | F | 2018 |
| 10 | Libby Marshall '90 | AA 53.83 | F | 1990 |
| 11 | Katie Swan '23 | 54.16 | F | 2020 |
| 12 | Mary Kate Turk '22 | 54.31 | F | 2019 |
| 13 | Katherine Sweetser '17 | 54.47 | F | 2014 |
| 14 | Sasha Newton '16 | 54.55 | F | 2016 |
| 15 | Elizabeth Tran '19 | 54.57 | F | 2019 |
| 16 | Teri Faller '11 | 54.61 | F | 2011 |
| 17 | Salena Casha '09 | 54.75 | F | 2007 |
| 18 | Hailey Novis '13 | 54.76 | F | 2010 |
| 19 | Sisi Ruan '07 | 55.13 | F | 2006 |
| 20 | Kerryn O'Connor '01 | 55.15 | F | 1998 |
| 21 | Miranda An '15 | 55.22 | F | 2015 |
| 22 | Devin Murphy '01 | 55.30 | F | 2000 |
| 23 | Sofia Smirnov '22 | 55.30 | F | 2020 |
| 24 | Juliette Farmer '18 | 55.35 | F | 2015 |
| 25 | Graeleigh Jones '21 | 55.35 | F | 2019 |